TABLE 3.

Effects of deletion of putative deacetylase gene RPD3 on frequency of switching in the white-opaque transition^a

Initial phenotype of strain	Genotype	No. of colonies (frequency [CFU])			Fold increase over TU17	No. of original-phase colonies with opposite-phase sectors (frequency [CFU])
		Total	White phase	Opaque phase
White
TU17	RPD3/RPD3	4,633	4,631	2 (4 × 10−4)		1 (2 × 10−4)
RPho19	rpd3/rpd3	2,104	2,073	31 (1 × 10−2)	25	66 (3 × 10−2)
RPho27	rpd3/rpd3	3,776	3,720	56 (2 × 10−2)	50	15 (4 × 10−3)
RPho19+27	rpd3/rpd3	5,880	5,793	87 (1 × 10−2)	25	71 (1 × 10−2)
Opaque
TU17	RPD3/RPD3	3,466	2 (6 × 10−4)	3,464		0 (<3 × 10−4)
RPho19	rpd3/rpd3	1,662	139 (5 × 10−2)	2,773	83	32 (1 × 10−2)
RPho27	rpd3/rpd3	2,398	24 (1 × 10−2)	2,374	16	8 (3 × 10−3)
RPho19+27	rpd3/rpd3	4,060	163 (3 × 10−2)	5,147	50	40 (1 × 10−2)

^aCells from white- or opaque-phase colonies were plated and incubated at 25°C for 5 days, and then the proportions of colony phenotypes (white, opaque, or sectored) were counted.