TABLE 3.
Effects of deletion of putative deacetylase gene RPD3 on frequency of switching in the white-opaque transitiona
| Initial phenotype of strain | Genotype | No. of colonies
(frequency [CFU])
|
Fold increase over TU17 | No. of original-phase colonies with opposite-phase sectors (frequency [CFU]) | ||
|---|---|---|---|---|---|---|
| Total | White phase | Opaque phase | ||||
| White | ||||||
| TU17 | RPD3/RPD3 | 4,633 | 4,631 | 2 (4 × 10−4) | 1 (2 × 10−4) | |
| RPho19 | rpd3/rpd3 | 2,104 | 2,073 | 31 (1 × 10−2) | 25 | 66 (3 × 10−2) |
| RPho27 | rpd3/rpd3 | 3,776 | 3,720 | 56 (2 × 10−2) | 50 | 15 (4 × 10−3) |
| RPho19+27 | rpd3/rpd3 | 5,880 | 5,793 | 87 (1 × 10−2) | 25 | 71 (1 × 10−2) |
| Opaque | ||||||
| TU17 | RPD3/RPD3 | 3,466 | 2 (6 × 10−4) | 3,464 | 0 (<3 × 10−4) | |
| RPho19 | rpd3/rpd3 | 1,662 | 139 (5 × 10−2) | 2,773 | 83 | 32 (1 × 10−2) |
| RPho27 | rpd3/rpd3 | 2,398 | 24 (1 × 10−2) | 2,374 | 16 | 8 (3 × 10−3) |
| RPho19+27 | rpd3/rpd3 | 4,060 | 163 (3 × 10−2) | 5,147 | 50 | 40 (1 × 10−2) |
a
Cells from white- or opaque-phase colonies were plated and incubated at 25°C for 5 days, and then the proportions of colony phenotypes (white, opaque, or sectored) were counted.