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| source parameters | ESI– | ESI+ |
|---|---|---|
| source gases 1 and 2 (psi) | 50 | 50 |
| curtain gas (psi) | 30 | 30 |
| source temperature (°C) | 275 | 250 |
| spray voltage (V) | –4500 | 5500 |
| CAD gas (psi) | 9 | 9 |
| scan cycle | 1759 | 1759 |
| IDA parameters | IDA, MS1/MS2 (−) | IDA, MS1/MS2 (+) |
|---|---|---|
| range (m/z) | 80–650/ 25–580 | 80–650/25–580 |
| declustering potential (V) | –80 ± 5/–50 ± 5 | 80 ± 5/ 70 ± 5 |
| CE (V) | –10/–20 ± 15 | 10/23 ± 15 |
| accumulation time (sec) | 0.1/0.05 | 0.1/0.05 |
| IDA intensity threshold (cps) | 500 | 500 |
