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| acquisition gradient: time (min), % organic, flow rate (mL/min) | (0.00, 20.0, 0.35) (3.00, 60.0, 0.35) (10.00, 99.9, 0.35) |
|---|---|
| rinse gradient: time (min), % organic, flow rate (mL/min) | (0, 20.0, 0.35) (0.01, 99.9, 0.53) (4.05, 99.9, 0.53) (4.10, 22.0, 0.45) (8.00, 20.0, 0.40) (9.00-10.00, 20.0, 0.35) |
