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. 2022 Aug 22;23(10):e53813. doi: 10.15252/embr.202153813

Figure 5. The lack of Lint‐O in flies leads to abnormal ovarian morphology and female sterility.

Figure 5

  1. CRISPR‐mediated generation of lint‐O knockout (KO). Genomic structure of lint‐O is shown with gRNA targeting lint‐O exon 1 (triangle). Sequences of lint‐O DNA in WT (y w) (complementary to the gRNA sequence) and the KO mutant, Lint‐O KO , are shown. PAM: protospacer adjacent motif (underlined). E1‐3: Exons 1–3. CDS: protein‐coding sequence. UTR: untranslated region.
  2. RT–qPCR analysis shows the mRNA levels of lint‐O in y w and Lint‐O KO ovaries. Data are expressed as mean and error bars represent SD. n = 2 biological replicates.
  3. The numbers of progeny in y w and Lint‐O KO are shown. Ten independent crosses were performed. Boxplot central bands, upper edges of boxes, lower edges of boxes, upper whiskers, and lower whiskers show median, third quartile, first quartile, maxima, and minima, respectively.
  4. Confocal images of y w and Lint‐O KO ovaries immunostained for Vasa. Scale bar: 50 μm.
  5. Confocal images of y w and Lint‐O KO ovarioles immunostained for Vasa. Vasa was ectopically expressed in follicle cells (white arrowheads). Scale bar: 50 μm.