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. 2022 Aug 18;23(10):e54605. doi: 10.15252/embr.202254605

Figure EV3. CRB3 exits the Golgi within RAB6+ vesicles.

Figure EV3

  • A
    SBP‐CRB3‐GFP and GalNacT2‐mCherry expression in aRG cells before and 45 min after addition of biotin. SBP‐CRB3‐GFP relocates from a diffuse perinuclear localization to the Golgi. Scale bar = 5 μm.
  • B
    SBP‐CRB3‐GFP and mCherry‐RAB6A localization in HeLa cells before and 40 min after addition of biotin. Scale bar = 5 μm. White arrowheads: colocalizing foci.
  • C
    Quantification of SBP‐CRB3‐GFP and mCherry‐RAB6A colocalization away from the Golgi apparatus 40 min after biotin addition. N = 16 cells from three independent experiments.
  • D
    SBP‐CRB3‐GFP and mCherry‐RAB6A localization in dissociated aRG cells cultivated in vitro, 40 min after addition of biotin. Scale bar = 5 μm. Yellow arrowheads: colocalizing foci.
  • E
    Quantification of SBP‐CRB3‐GFP and mCherry‐RAB6A colocalization away from the Golgi apparatus 40 min after biotin addition. N = 14 cells from three independent experiments.
  • F
    SBP‐CRB3‐GFP and mCherry‐RAB6A localization in aRG cells cultivated within brain slices, 40 min after addition of biotin. Scale bar = 5 μm. Yellow arrowheads: colocalizing foci.
  • G
    Quantification of SBP‐CRB3‐GFP and mCherry‐RAB6A colocalization away from the Golgi apparatus 40 min after biotin addition. N = 15 cells from three independent experiments.

Data information: All error bars indicate SD.