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. 2022 Aug 18;23(10):e54605. doi: 10.15252/embr.202254605

Figure 4. Post‐Golgi apical transport of Crumbs is driven by dynein.

Figure 4

  • A
    Immuno‐staining for Crumbs3 (CRB3) and PALS1 in E15.5 embryonic cortex. Scale bar = 25 μm.
  • B
    Schematic representation of the RUSH system. CRB3 is retained in the endoplasmic reticulum (ER) until the addition of biotin, which releases it for trafficking. SBP: Streptavidin‐binding protein. St: Streptavidin.
  • C
    RUSH assay for CRB3‐GFP in control (mcherry) and dynactin‐inhibited radial glial cells (CC1‐p150‐dsRed), electroporated at E.15.5 and imaged at E16.5. Scale bar = 5 μm.
  • D
    CRB3 localization at the apical surface upon release.
  • E
    Percentage of cells with 100% of CRB3 signal at the apical surface upon release.

Data information: (D, E) mCherry (control): N = 3 brains per timepoint (361 cells total). CC1‐p150: N = 3 brains per timepoint (2 brains for T0) (268 cells total). Fisher's exact test and Benjamini–Hochberg procedure, ***P ≤ 0.001. All error bars indicate SD.