Figure 5. LIS1 knockout leads to ectopically dividing progenitors.

• A
  PAX6 and phospho‐Histone 3 (p‐H3) staining in WT and LIS1 KO E12.5 brains. Cortices were subdivided into five bins of equal size along the radial axis. Scale bar = 25 μm.
• B
  Percentage of PH3+/PAX6+ cells located above the ventricular surface of WT and LIS1 KO E12.5 brains. WT: 1192 cells from N = 6 brains. LIS1 KO: 589 cells from N = 3 brains.
• C
  Phospho‐Vimentin (p‐VIM) staining in WT and LIS1 KO E12.5 brains. Scale bar = 25 μm.
• D
  Percentage p‐VIM+ cells dividing ectopically, away from the ventricular surface of WT and LIS1 KO E12.5 brains. WT: 1056 cells from N = 6 brains. LIS1 KO: 879 cells from N = 3 brains.

Data information: (B, D) Mann–Whitney U test, *P ≤ 0.05. All error bars indicate SD.