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. 2022 Aug 18;23(10):e54605. doi: 10.15252/embr.202254605

Figure 6. RAB6A/B and LIS1 are required for Crumbs localization and junctional integrity.

Figure 6

  • A
    CRB3 and PALS1 staining in WT, RAB6A/B dKO (E15.5), and LIS1 KO (E12.5) brains. Scale bar = 25 μm.
  • B
    Quantification of CRB3 staining interruptions along the ventricular boundary of WT (E12.5 and E15.5; N = 13 brains), LIS1 KO (E12.5, N = 4 brains), and RAB6A/B dKO (E15.5, N = 4 brains).
  • C
    N‐Cadherin staining in WT, RAB6A/B dKO (E15.5) and LIS1 KO (E12.5) brains. Scale bar = 25 μm.
  • D
    Quantification of N‐Cadh staining interruptions along the ventricular boundary of WT (E12.5 and E15.5; N = 6 brains), LIS1 KO (E12.5, N = 3 brains), and RAB6A/B dKO (E15.5, N = 3 brains).
  • E
    Model for post‐Golgi apical transport of CRB3 to the apical surface of aRG cells.

Data information: (B, D) Kruskal–Wallis test with a Dunn post‐hoc test and Benjamini–Hochberg procedure, *P ≤ 0.05, ***P ≤ 0.001. All error bars indicate SD.