Fig. 2.

Evaluation of the viral load in SARS-CoV-2 infected mice treated with nirmatrelvir, remdesivir and molnupiravir monotherapies, their combinations, nirmatrelvir-remdesivir and nirmatrelvir-molnupiravir. (A) Lungs and brains were collected at 5 DPI, and viral titers were estimated. The viral titer is expressed in log₁₀ PFU/mL, and the limit of detection is shown in a dotted line (one-way ANOVA with Tukey's multiple comparison test; *p < 0.05, **p < 0.01). (B) Viral RdRp gene quantification in both the lungs and brain at 5 DPI was determined via RT–qPCR and results are expressed in 40-Ct (One-way ANOVA with Tukey's multiple comparison test; *p < 0.05, **p < 0.01, ***p < 0.001). (C) Viral N gene quantification in both the lung and brain at 5 DPI via RT–qPCR. The data are expressed in 40-Ct (one-way ANOVA with Tukey's multiple comparison test; *p < 0.05, **p < 0.01, ***p < 0.001).