TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| R. sphaeroides | ||
| 2.4.1 | Wild type | Laboratory strain |
| CCMA1 | ccmA1::Ωspc | This work |
| CCMA2 | ccmA1::Ωspc-pSUP202 (Tcr; merodiploid) | This work |
| CCMA3 | ccmA2::pLO1 (Knr sucroses; merodiploid) | This work |
| CCMA4 | ΔccmA because it contains the ccmA2 allele | This work |
| CCMG2 | ccmG1::Ωspc-pSUP202 (Tcr; merodiploid) | This work |
| E. coli | ||
| DH5α | Plasmid maintenance | 1 |
| S17-1 | Donor for plasmid transfer to R. sphaeroides | 27 |
| Plasmids | ||
| p2-9 | R. capsulatus ccmABC | 12 |
| pCSP4 | Apr; ∼9.5-kb PstI ccmABCDG fragment cloned in pUC18 in opposite orientation as Plac | This work |
| pCSP20 | Apr; ∼3.5-kb BamHI ccm fragment cloned in pUC18 in opposite orientation as Plac | This work |
| pCSP20Ω1 | Apr; pCSP20 containing spc cloned into ccmA (source of ccmA1::spc allele) | This work |
| pCSP91 | Apr Spr; ∼5.5-kb BamHI fragment containing ccmA1::spc from pCSP20Ω1 cloned in pSup202 | This work |
| pHP45Ω | Source of spc gene | 7 |
| pLO1 | Knr; sacB; RP4 oriT; ColE1 ori | 14 |
| pRC10 | Knr; ∼1.5-kb fragment containing ccmA2 cloned into the Pmel site of pLO1 | This work |
| pRK4S | Tcr; ∼9.5-kb PstI fragment containing ccmABCDG cloned into pRK415 | This work |
| pRK415 | Tcr; broad-host-range plasmid | 11 |
| pSUP202 | Apr Tcr Cmr Mob+; pBR325 derivative | 27 |
| pTP1 | Apr; ∼11.5-kb PstI fragment containing ccmG1::spc in pUC18 | This work |
| pTP2 | Tcr; Spr; ccmG1::spc from pTP1 cloned into PstI site of pSUP202 | This work |
| pUI8298 | Tcr; ∼29-kb fragment with ccmABCDG on pLA2917 | This work |
| pUC18 | Apr; cloning vector | 32 |