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Use of a merodiploid to generate null mutations at ccmABCDG or cycA
| Strain/gene | No. of colonies found with genotype:
|
||
|---|---|---|---|
| Loss of function (Tcs) | Merodiploid (Spr Tcr) | Wild type (Sps Tcs) | |
| CYCA2/cycA | 24,913 | 42 | 45 |
| CCMG2/ccmG | 0 | 39,985 | 15 |
| CCMA2/ccmA | 0 | 19,956 | 44 |
