Skip to main content
. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Cell Signal. 2022 Feb 24;93:110294. doi: 10.1016/j.cellsig.2022.110294

Figure 5 -. GPR65 knockdown in macrophages alters phagocytosis.

Figure 5 -

THP-1 cells were transduced with two different GPR65 shRNAs or empty vector (EV) control. PMA-differentiated THP-1 cells were placed in two different pHs (6.5 and 8.0) for four hours, with cytochalasin D or DMSO for the last hour. Cells were then incubated for one hour with pH sensitive pHRodo E. coli (A) GPR65 KD and pH effect on the mean fluorescence intensity (MFI) of positive cells. (B) GPR65 KD and pH effect on the percentage of positive phagocytic cells. Barplots represent geometric mean with SE. Detailed effect sizes analyses from ANOVA are presented in Table S4.