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[Preprint]. 2022 Sep 26:2022.09.24.509341. [Version 1] doi: 10.1101/2022.09.24.509341

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Extended Data Fig. 5 | — a, SDS-PAGE gels of soluble spike proteins after His-tag affinity purification. Molecular weights (in kDa) of protein standards are shown. Arrowheads point to the band corresponding to the size of a glycosylated S monomer (~180 kDa). b, Chromatograms of soluble spike ectodomain mutants for biophysical characterization. Dotted line indicates the peak volume (~74 mL) corresponding to the size of a glycosylated spike trimer (~540 kDa). Fractions from 73 mL to 79 mL were collected and concentrated. c, Culture volume and yield for each mutant in b. Abbreviations for combinatorial mutations are as follows: QR, D994Q/Q1005R; 2P, K986P/V987P; 2PQ, K986P/V987P/D994Q; 2PR, K986P/V987P/Q1005R; 2PQR, K986P/V987P/D994Q/Q1005R. Source data are available.