Extended Data Table 1.

Mutations that are included in our DMS library (residues 883 to 1034) and also found in SARS-CoV-2 variants of concern and variants of interest.

Variants of concern
PANGO Lineage	Mutations	GenBank Reference
Alpha (B.1.1.7)	S982A	MZ344997.1
Beta (B.1.351)	-	MW598419.1
Gamma (P.1)	T1027I	MZ169911.1
Delta (B.1.617.2)	D950N	MZ359841.1
Omicron (B.1.1.529: BA.1)	Q954H, N969K, L981F	OL672836.1
Omicron (B.1.1.529: BA.2, BA.4, BA.5)	Q954H, N969K	OM685375.1 (BA.2) ON373214.1 (BA.4) ON249995.1 (BA.5)
Variants of interest
PANGO Lineage	Mutations	GenBank Reference
Epsilon (B.1.427, B.1.429)	-	MW453103.1
Zeta (P.2)	-	MW523796.1
Eta (B.1.525)	F888L	MW560924.1
Iota (B.1.526)	-	MW643362.1
Kappa (B.1.617.1)	-	MW966601.1
Lambda (C.37)	-	MW850639.1
Mu (B.1.621)	D950N	EPI_ISL_1220045 (GISAID)