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. 2022 Oct 6;13:113. doi: 10.1186/s40104-022-00762-8

Table 4.

The qPCR validation of the RNA-seqa

Genesb RNA-seqc (LFM vs. CON) Real-time PCRd (LFM vs. CON)
FC P-value FC P-value
TNFRSF13C 0.46 0.037 0.36 0.048
CXCL10 0.50 0.024 0.42 0.026
FABP1 8.77 0.002 8.36 0.034
LTA 0.43 0.030 0.45 0.087
LYZ1 7.01 0.007 7.05 0.026
PIGR 6.02 0.013 6.14 0.042
SLC5A8 2.02 0.022 2.69 0.035
TFF2 5.06 0.001 5.02 0.004

aData were presented as FC. The gene expressions were considered to be significantly altered when FC > 2 or < 0.5, P < 0.05

bTNFRSF13C: tumor necrosis factor receptor superfamily member 13C; CXCL10: C-X-C motif chemokine 10; FABP1: fatty acid binding protein 1; LTA: lymphotoxin alpha; LYZ1: lysozyme-like protein 1; PIGR: polymeric immunoglobulin receptor; SLC5A8: sodium-coupled monocarboxylate transporter; TFF2: trefoil factor 2

cResults based on RNA sequencing, n = 4

dResults based on real-time PCR, n = 6