TABLE 1.
Expression of aprE′-′lacZ in B. subtilis strains carrying either multicopy degR or degU32(Hy) and degS200(Hy) mutations
| Expt | Straina | Relevant genotype | β-Galactosidase activityb | Stimulationc |
|---|---|---|---|---|
| 1 | YY102(pNC6) | relA+ | 107 | 1 |
| YY102(pNC61) | relA+ multicopy degR | 10,330 | 96 | |
| HT1013(pNC6) | relA | 18 | 1 | |
| HT1013(pNC61) | relA multicopy degR | 4,650 | 258 | |
| 2 | YY102 | relA+ | 133 | 1 |
| YY202 | relA+degU32(Hy) | 9,380 | 70 | |
| YY299 | relA+degS200(Hy) | 7,260 | 54 | |
| HT1013 | relA | 20 | 1 | |
| HT1020 | relA degU32(Hy) | 1,020 | 51 | |
| HT1022 | relA degS200(Hy) | 820 | 41 |
Plasmids carried by the host strains are shown in parentheses. For strains YY102 and HT1013, refer to the legend to Fig. 1. Strains YY202 [trpC2 degU32(Hy) aprE::pSKK25] and YY299trpC2 [degS200(Hy) aprE::pSKK25] are the degU32(Hy) and degS200(Hy) derivatives, respectively, of YY102. Strains HT1020 [trpC2 degU32(Hy) relA::tet aprE::pSKK25] and HT1022 [trpC2 degS200(Hy) relA::tet aprE::pSKK25] are the degU32(Hy) and degS200(Hy) derivatives, respectively, of HT1013.
β-Galactosidase activities in Miller units were measured at T0 through T5 as shown in Fig. 1, and the values shown are the peak values observed during this period.
The values show the extent of stimulation caused by multicopy degR, degU32(Hy), and degS200(Hy) mutations relative to the control level, which was set as unity.