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. 2022 Jun 8;42(10):1920–1932. doi: 10.1177/0271678X221105683

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Figure 1. — BACE1 regulated eNOS expression and NO production in human BMECs. A-F: Cells were treated with BACE1siRNA or CtsiRNA (30 nM) for 48 hours (a and c–f) or 40 hours (b), Western blot or real-time PCR were performed, respectively. a, BACE1 protein was knocked down using BACE1siRNA, n = 8 *P < 0.05. b, levels of eNOS mRNA were increased in cells treated with BACE1siRNA, n = 4, *P < 0.05. c–f, protein expressions of eNOS, p-eNOS(S1177)/eNOS, and p-eNOS(T495)/eNOS) in human BMECs treated BACE1siRNA or CtsiRNA, n = 15-24, *P < 0.05. g and h: Human BMECs were transduced with Ad-BACE1 or Ad-Null (2.5 MOI, 2 days). g, n = 9, H, n = 13; *P < 0.05. i: Human BMECs were treated with BACE inhibitor IV for 24 hours (n = 5, *P < 0.05). J: After endothelial cells were treated with 30 nM BACE1siRNA for 2 days, cells were incubated with EGM2 without serum for 24 hours. The supernatants were collected for NO₂+NO₃ assay (n = 8, *P < 0.05).

Figure 1. — BACE1 regulated eNOS expression and NO production in human BMECs. A-F: Cells were treated with BACE1siRNA or CtsiRNA (30 nM) for 48 hours (a and c–f) or 40 hours (b), Western blot or real-time PCR were performed, respectively. a, BACE1 protein was knocked down using BACE1siRNA, n = 8 *P < 0.05. b, levels of eNOS mRNA were increased in cells treated with BACE1siRNA, n = 4, *P < 0.05. c–f, protein expressions of eNOS, p-eNOS(S1177)/eNOS, and p-eNOS(T495)/eNOS) in human BMECs treated BACE1siRNA or CtsiRNA, n = 15-24, *P < 0.05. g and h: Human BMECs were transduced with Ad-BACE1 or Ad-Null (2.5 MOI, 2 days). g, n = 9, H, n = 13; *P < 0.05. i: Human BMECs were treated with BACE inhibitor IV for 24 hours (n = 5, *P < 0.05). J: After endothelial cells were treated with 30 nM BACE1siRNA for 2 days, cells were incubated with EGM2 without serum for 24 hours. The supernatants were collected for NO₂+NO₃ assay (n = 8, *P < 0.05).