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. 2022 Jun 24;4(10):648–661. doi: 10.1096/fba.2021-00161

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© 2022 The Authors. FASEB BioAdvances published by Wiley Periodicals LLC on behalf of The Federation of American Societies for Experimental Biology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Phenotypic differences of C57BL/6, FVB/N, and BALB/c Tregs. (A) Foxp3 expression of splenic CD4⁺ cells of C57BL/6, FVB/N, and BALB/c mice. Freshly isolated splenocytes were stained and analyzed. Expression was analyzed using flow cytometry. Lymphocytes were gated based on forward scatter/side scatter and then subsequently gated on CD4⁺ cells. (B) Expression of various Treg markers of CD4⁺Foxp3⁺ cells. Expression was analyzed using flow cytometry. Lymphocytes were gated based on forward scatter/side scatter and then subsequently gated on CD4⁺Foxp3⁺ cells. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Data are representative of 3 independent experiments, with 4 samples per group. Mean + standard deviation is shown.