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. 2022 Jun 24;4(10):648–661. doi: 10.1096/fba.2021-00161

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© 2022 The Authors. FASEB BioAdvances published by Wiley Periodicals LLC on behalf of The Federation of American Societies for Experimental Biology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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RNA expression of various Treg markers. (A) mRNA expression of Treg markers on CD4⁺CD25⁻ Teff cells. Cells were cultured for 2 days with anti‐CD3 and anti‐CD28 stimulation to expand cell numbers. RNA was isolated, cDNA generated, and expression analyzed. (B) mRNA expression of Treg markers on CD4⁺CD25⁺ Treg cells. Cells were cultured for 2 days to expand cell numbers. RNA was isolated, cDNA generated, and expression analyzed. (C) Comparison of mRNA expression of each strain's Tregs relative to Teff cells. *p ≤ 0.05; **p ≤ 0.01; ***P ≤ 0.001. Data are representative of 2 independent experiments, with 4 samples per group. Mean + standard deviation is shown.