FIG. 3.

Recombinant spvBC complements virulence plasmid-cured serovar Typhimurium after s.c. inoculation of mice. Mice were inoculated s.c. with wild-type strain χ3306 or virulence plasmid-cured strain χ3337 containing spvBC-bearing pGTR356, spvRABCD-bearing pGTR061, or no plasmid. Four days later, spleens were removed, homogenized, and plated to enumerate CFU. P values are for χ3337(pGTR356) being different from the other strains. Additionally, χ3456 and χ3337(pGTR061) were each significantly greater than χ3337 (P < 0.0001), and χ3337(pGTR061) was significantly greater than χ3456 (P < 0.02). Error bars represent standard deviations. n = 5 (for all groups).