FIG. 4.
spvC is required for virulence conferred by spvBC-bearing pGTR356. Mice were inoculated s.c. with 105 CFU of either wild-type χ3306, virulence plasmid-cured χ3337, χ3337(pGTR356), or χ3337(pGTR357). Six days later, spleens were removed, homogenized, and plated to enumerate CFU. The spvC mutation of pGTR357(∗) significantly decreased infection, compared with spvBC-bearing pGTR356 (P < 0.01). N.S., χ3306 was not significantly different from χ3337(pGTR356) (P > 0.3). Error bars represent standard deviations. n = 5 (for all groups).