TABLE 2.
Expression of recombinant spvC can complement lack of spvD to confer virulence to serovar Typhimurium in mice
| Plasmid used to transform χ3337(pGTR040) | CFU ofab:
|
Paired differencea | P valuec | |
|---|---|---|---|---|
| χ3337(pGTR040) | χ3456 | |||
| pGTR153 | 5.5 ± 1.2 | 5.1 ± 1.2 | −0.4 ± 1.1 | >0.35 |
| pGTR127 | 4.8 ± 0.8 | 5.0 ± 1.0 | 0.2 ± 0.6 | >0.35 |
| pACYC184 | 4.1 ± 1.4 | 5.2 ± 1.6 | 1.1 ± 0.5 | <0.005 |
a
Means ± standard deviations of log-transformed data are shown. n = 6 (for all groups).
b
Virulence plasmid-cured serovar Typhimurium χ3337(pGTR040), which carries spvRABCD′, was transformed with either pGTR153 (carrying only spvD), pGTR127 (carrying only spvC), or the vector pACYC184. These strains were mixed with wild-type serovar Typhimurium χ3456 and inoculated orally to mice at a dose of 108 CFU. Five days later, spleens were harvested and plated to enumerate CFU of each strain.
c
P value for the paired difference being different from 0.