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. 2022 Sep 8;7(17):e161045. doi: 10.1172/jci.insight.161045

Figure 2. Aged people show a lower and less polyfunctional SARS-CoV-2 S–specific CD4+ and CD8+ T cell response after the vaccination.

Figure 2

(A and B) Dot plots presenting the percentage of IFN-γ+, CD107a+, and PRF+ cells within memory, CM, EM, and TEMRA CD4+ (A) and CD8+ (B) T cells after S-specific SARS-CoV-2 stimulation, comparing participants > 60 years old (red) and < 60 years old (blue) 3 weeks after the first dose (1D) and 2 months after the second dose (2D) of SARS-CoV-2 vaccine (right). Pseudocolor dot plot graphs show a representative data of memory CD4+ T cells from a > 60-year-old and a < 60-year-old donor 2 months after vaccination (left). (C) Pie charts representing SARS-CoV-2 S–specific memory CD4+ T cell polyfunctionality. Each sector represents the proportion of S-specific CD4+ T cells producing 2 (green) or 1 (blue) functions. Arcs represent the type of function (CD107a, IFN-γ, IL-2, PRF, and TNF-α) expressed in each sector. (D) Bar graphs showing the percentage of EM and CM CD4+ T cells expressing different combinations of studied functions (CD107a, IFN-γ, IL-2, PRF, and TNF-α) comparing > 60-year-old (red) and < 60-year-old (blue) participants after the first (1D) and the second (2D) dose. Mann-Whitney U (A, B, and D), Wilcoxon (A, B, and D), and Permutation (C) tests were used (n = 41). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.