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. 2001 Aug;183(15):4659–4663. doi: 10.1128/JB.183.15.4659-4663.2001

FIG. 1.

FIG. 1

Characterization of the pstS mutation in C86. (A) Amplification of the pstS gene and its promoter region by PCR using chromosomal DNA of strains K10, C86, C78, and MC4100 (5) and the primers pst4 (5′-GAGTAATAAATGGATGCCC-3′) and pst5 (5′-CGGTGGGTTAAAAGCAGGC-3′). Strains K10 (pitA10), C86 (pstS21 derivative of K10), and C78 (pstS28 derivative of K10) were kindly provided by the E. coli Genetic Stock Center (Department of Biology, Yale University, New Haven, Conn.). The positions of the molecular size markers are indicated at the left. (B) Position of the IS2 element in the promoter region of the pstS gene in strain C86. The two pho boxes, the −10 region and the Shine-Dalgarno sequence (SD), are indicated. The start of the coding region of pstS is indicated by an arrow.