FIG. 1.
Effect of zinc on growth E. coli W3110 (□), RW3110 (zntA::Km) (▴), GG48 (ΔzitB::Cm zntA::Km) (■), and GG48 (ΔzitB::Cm zntA::Km)/pZITB (⧫). Growth curves with different ZnCl2 concentrations are shown. Overnight cultures were diluted 1:500 into fresh Luria-Bertani medium with the indicated concentrations of ZnCl2. Cell growth was monitored as the optical density at 600 nm after 15 h of incubation at 37°C with shaking and converted to dry weight. Experiments were performed in triplicate, values are averages.