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. 2022 Oct 6;17(10):e0274993. doi: 10.1371/journal.pone.0274993

Fig 1. PfRSM22 and PfMRPL23 are localized to the mitochondrion and are essential for parasite survival.

Fig 1

(A) Images from fluorescence microscopy showing colocalization of PfRSM22-3HA (green) with Mitotracker (red). (B) Colocalization of PfMRPL23-3HA (green) with Mitotracker (red). In A and B, the parasite nucleus is stained using DAPI (blue). (C) Western blot showing expression of PfRSM22-3HA protein (62 KDa) in the presence or absence of 250 nM aTc over 6 days (3 IDCs). (D) Western blot showing expression of PfMRPL23-3HA protein (32 KDa) in the presence or absence of 250 nM aTc over 4 days (2 IDCs). Pf-Exp2 (33 KDa) was used as a loading control. Representative images of Giemsa-stained D10-PfRSM22-3HA parasites (E) and D10-PfMRPL23-3HA parasites (F) at the trophozoite stage in the presence (aTc ON) and absence of aTc (aTc OFF). Quantification of D10-PfRSM22-3HA parasite growth (G) and D10-PfMRPL23-3HA parasite growth (H) over 8 days in the presence and absence of aTc. Growth index was calculated by multiplication of parasitemia with splitting factors over the time course. Data shown here is mean ± SD of n = 3.