Video 1. ICAM-based adhesion yields reversible LOV2 binding for optogenetically stimulated cells.

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Time course of LOV2 on a supported lipid bilayer reversibly binding zdk-CARs expressed in Jurkat cells in the presence of ICAM-1 adhesion to the bilayer (light-independent cellular interaction). LOV2 is released from cell footprint under blue light and rebinds in the dark (illumination status indicated in top left). Cells were adhered to the bilayer for 5 min prior to the start of the video. Two minutes of strong blue-light illumination are followed by 3 min in the dark for two cycles (MM:SS time-stamp in top right).