Video 4. ICAM-based adhesion yields reversible diacylglycerol (DAG) generation for optogenetically stimulated cells.

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Time course of reversible DAG generation in Jurkat cells in the presence of ICAM-1 adhesion to the bilayer (light-independent cellular interaction). DAG is generated during active antigen signaling in the dark, and DAG generation is terminated following blue-light-mediated dissociation of the receptor-ligand interaction (illumination status indicated in top left). Cells adhered to the bilayer for 5 min prior to the start of the video. Two minutes of strong blue-light illumination are followed by 3 min in the dark for two cycles (MM:SS time-stamp in top right).