Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (C. elegans) | C. elegans wild isolate | Caenorhabditis Genetics Center (CGC) | N2 | |
| Strain, strain background (C. elegans) | atg-18(gk378) V | CGC | VC893 | |
| Strain, strain background (C. elegans) | atg-13(bp414) III | CGC | HZ1688 | |
| Strain, strain background (C. elegans) | pdr-1(gk448) III | CGC | VC1024 | |
| Strain, strain background (C. elegans) | xnSi1 [mex-5p::GFP-PH::nos-2 3’UTR, unc-119(+)] II; unc-119(ed3) III | Chihara and Nance, 2012 | FT563 | |
| Strain, strain background (C. elegans) | hmg-5(xn107[hmg-5-GFP]) IV | This study | FT2064 | hmg-5(xn107) made by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR). |
| Strain, strain background (C. elegans) | hmg-5(xn107[hmg-5-GFP]) IV; xnIs360 [pMRR08(mex-5p::mCherry-PH::nos-2 3’UTR, unc-119(+))] V | This study | FT2133 | Shown in Figure 1J–K. |
| Strain, strain background (C. elegans) | xnSi67 [pYA57(mex-5p::mito-tomm-201-54-Dendra2::nos-2 3’UTR, unc-119(+))] I; unc-119(ed3) III | This study | FT1885 | Made by Mos1-mediated single copy insertion (MosSCI). |
| Strain, strain background (C. elegans) | xnSi67 [pYA57(mex-5p::mito-tomm-201-54-Dendra2::nos-2 3’UTR, unc-119(+))] I; xnIs360 [pMRR08(mex-5p::mCherry-PH::nos-2 3’UTR, unc-119(+))] V | This study | FT1900 | Shown in Figure 1B–D, Figure 1—figure supplement 1, and Figure 2C–D. |
| Strain, strain background (C. elegans) | xnSi67 [pYA57(mex-5p::mito-tomm-201-54-Dendra2::nos-2 3’UTR, unc-119(+))] I; xnSi45 [pYA11(mex-5p::mCherry-moma-1::nos-2 3’UTR, unc-119(+))] II | This study | FT2366 | Shown in Figure 1G and Figure 2—figure supplement 4. |
| Strain, strain background (C. elegans) | xnSi67 [pYA57(mex-5p::mito-tomm-201-54-Dendra2::nos-2 3’UTR, unc-119(+))] I; xnSi45 [pYA11(mex-5p::mCherry-moma-1::nos-2 3’UTR, unc-119(+))] II; uaDf5 /+ mtDNA | This study | FT2414 | Shown in Figure 5E. |
| Strain, strain background (C. elegans) | xnSi67 [pYA57(mex-5p::mito-tomm-201-54-Dendra2::nos-2 3’UTR, unc-119(+))] I; xnSi45 [pYA11(mex-5p::mCherry-moma-1::nos-2 3’UTR, unc-119(+))] II; atg-18(gk378) V; uaDf5 /+ mtDNA | This study | FT2417 | Shown in Figure 5F. |
| Strain, strain background (C. elegans) | xnSi73 [mex-5p::GFP1-10::nos-2 3’UTR, unc-119(+)] I; xnSi45 [pYA11(mex-5p::mCherry-moma-1::nos-2 3’UTR)] II | This study | FT2128 | xnSi73 made by CRISPR, see Methods. |
| Strain, strain background (C. elegans) | xnSi85 [mex-5p::mito(matrix)-GFP1-10::nos-2 3’UTR] I; xnSi45 [pYA11(mex-5p::mCherry-moma-1::nos-2 3’UTR, unc-119(+))] II | This study | FT2293 | xnSi85 made by CRISPR, see Methods |
| Strain, strain background (C. elegans) | xnSi85 [mex-5p::mito(matrix)-GFP1-10::nos-2 3’UTR] I; xnSi45 [pYA11(mex-5p::mCherry-moma-1::nos-2 3’UTR, unc-119(+))] II; hmg-5(xn168[hmg-5-GFP11]) IV | This study | FT2296 |
hmg-5(xn168) made by CRISPR. Shown in Figure 3A–F, and Figure 3—figure supplement 1A, B |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II | This study | FT2279 |
glh-1(sam24) a gift from Dustin Updike (MDI Biological Laboratory) (Marnik et al., 2019). Base strain used for all cell sorting. Related to 2—6. |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; uaDf5 /+ mtDNA | This study | FT2283 | Related to data shown in Figure 4 |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; hmg-5(xn107[hmg-5-GFP]) IV | This study | FT2312 | Related to data shown in Figure 2. |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; nop-1(full CRISPR deletion) III | This study | FT2323 |
nop-1 deletion a gift from Heng-Chi Lee (University of Chicago) (Zhang et al., 2018). Related to data shown in 2–3. |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; nop-1(full CRISPR deletion) III; uaDf5 /+ mtDNA | This study | FT2332 | Related to data shown in Figure 4C–E |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; atg-18(gk378) V; uaDf5 /+ mtDNA | This study | FT2347 | Related to data shown in Figure 5 |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; atg-13(bp414) III; uaDf5 /+ mtDNA | This study | FT2402 | Related to data shown in Figure 5 |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; nop-1(full CRISPR deletion) III; atg-18(gk378) V | This study | FT2443 | Related to data shown in Figure 2 |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; pdr-1(gk448) III; uaDf5 /+ mtDNA | This study | FT2364 | Related to data shown in Figure 6 |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; pink-1(xn199[pink-1(STOP-IN)]); xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; uaDf5 /+ mtDNA | This study | FT2432 |
pink-1(xn199) made by CRISPR. Related to data shown in Figure 6. |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; pink-1(xn199[pink-1(STOP-IN)]); xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; pdr-1(gk448) III; uaDf5 /+ mtDNA | This study | FT2378 | Related to data shown in Figure 6. |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; mptDf2 /+ mtDNA | This study | FT2387 | Related to data shown in Figure 4—figure supplement 1. |
| Strain, strain background (C. elegans) | glh-1(sam24[glh-1-GFP-3xFLAG]) I; xnIs510 [pYA12(ehn-3p::mCherry-PH, unc-119(+))] II; dct-1(xn192[dct-1(STOP-IN)]) X uaDf5 /+mtDNA | This study | FT2339 |
dct-1(xn192) made by CRISPR. Related to data shown in Figure 5—figure supplement 1. |
| Strain, strain background (C. elegans) | xnSi67 [pYA57(mex-5p::mito(tomm-201-54)-Dendra2::nos-2 3’UTR)] I; xnSi45 [pYA11(mex-5p::mCherry-moma-1::nos-2 3’UTR, unc-119(+))] II; uaDf5 /+ mtDNA | This study | FT2414 | Shown in Figure 5E. |
| Strain, strain background (C. elegans) | xnSi67 [pYA57(mex-5p::mito(tomm-201-54)-Dendra2::nos-2 3’UTR)] I; xnSi45 [pYA11(mex-5p::mCherry-moma-1::nos-2 3’UTR, unc-119(+))] II; atg-18(gk378) V; uaDf5 /+ mtDNA | This study | FT2417 | Shown in Figure 5F. |
| Sequence-based reagent | ocrAS_Dendra-C-term | Integrated DNA Technologies (IDT) |
GTCCTCTACCAAGTCAAGCA | crRNA to replace Dendra in xnSi67 |
| Sequence-based reagent | ocrAS_Dendra-N-Term | IDT | AGAATGTCGGACACAATTCT | crRNA to replace Dendra in xnSi67 |
| Sequence-based reagent | ocrAS01 | IDT | AAGGGAGAAGAATTATTTAC | crRNA used to add MLS to GFP1-10 in xnSi73 |
| Sequence-based reagent | ocrAS13 | IDT | ATCTGCATTTTCTTTCTGTT | crRNA used for hmg-5 C-terminal tagging |
| Sequence-based reagent | ocrAS19 | IDT | GGTGATAAATGGGTTTGAGA | crRNA used for dct-1(STOP-IN) |
| Sequence-based reagent | ocrAS20 | IDT | CAGGTGTACTCTCGGTCAAT | crRNA used for dct-1(STOP-IN) |
| Sequence-based reagent | ocrAS25 | IDT | AACTCCTAAATTATAAGTGG | crRNA used for pink-1(STOP-IN) |
| Sequence-based reagent | ocrAS26 | IDT | ATGAACTCCTAAATTATAAG | crRNA used for pink-1(STOP-IN) |
| Sequence-based reagent | oAS115 | IDT | TTTATCGATAATCAATTGA ATGTTTCAGACAGAGAAT GGCACTCCTGCAATCAC GTCTCCTCCTGTCCGCC CCACGTCGTGCCGCCG CCACCGCCCGTGCCGG AGCTGGTGCAGGCGCT GGAGCCGGAGCCATGT CTAAGGGAGAAGAACT CTTCACTGGAGTTGTT CCTATCCTCGTCGAGC TCGACGGAGACG |
MLS-GFP1-10 repair template |
| Sequence-based reagent | oAS187 | IDT | tttgattacaaaatggaaag ttgtgacgaattcaaCTAG GTGATTCCGGCGG CATTGACATACTCA TGGAGGACCATGT GGTCACGTCCTCC TGAACCTCCTTGAT CTGCATTTTCTTTT TGTTCTGCTTCCC ATTTCTGGAGGAC GACATGGTATTCATCT |
hmg-5-GFP11 repair template |
| Sequence-based reagent | oAS216 | IDT | aaaaagtaaaacaaac CAGGTGTACTCT CGGTCAAGCTAG CTTATCACTTAGT CAAGCATAATCTG GAACATCATATGG ATAAGCGTAGTCT GGAACGTCGTATG GATATGCATAGTCT GGCACGTCGTATG GGTAGACGGCTTT TGCGGATGGTGTT GTCTGTTGAGCCG |
dct-1(STOP-IN) repair template |
| Sequence-based reagent | oAS245 | IDT | GAGCCTTTTTGAG TACGACATGAACT CCTAAATTAGCTA GCTTATCACTTAG TCACCTCTGCTCT GGACAAACTTCCC TCCTCCTGAACCT CCCGATGCTCCTG AGGCTCCCGATGC TCCTAAGTGGCGG GAAATATTCTCGGC AGGAAGCGTTG |
pink-1(STOP-IN) repair template |