FIG. 2.

P. furiosus contains a cellobiose binding protein. (A) [³H]cellobiose binding to membranes derived from cells grown on cellobiose (lane 1) or maltose (lane 2). Binding studies were performed as described. (B) Coomassie brilliant blue-stained SDS-PAGE gel. Comparison between membranes derived from maltose-grown cells (lane 1) and membranes derived from cellobiose-grown cells (lane 2). (C) Coomassie brilliant blue-stained gel of purification of CbtA. Lane 1, membranes; 2, ConA fraction; 3, purified CbtA.