Fig. 4.

LRG1 expression associates with IL-1 signaling and is KD-specific. A, B Among all molecules with an immunological signaling capability (cytokines, chemokines) assessed in KAWAKINRA study samples, LRG-1 serum levels associate particularly with IL-1β. C, D Median MFI expression levels (C) or absolute concentrations (D) of indicated markers into culture supernatants of human neutrophils (n = 4 independent donors), human coronary artery endothelial cells (HCAECs, 4 independent experiments), or fresh human whole blood (4 independent donors) following stimulation (4 h) with indicated concentrations of recombinant human IL-1b. E LRG1 expression (Z score of log10-transformed raw data, GSE63881) in IVIG responders (R, n = 220) and non-responders (NR, n = 121). F Expression of indicated genes (Z score of log10-transformed raw data, GSE63881) in paired samples of IVIG-resistant KD patients during acute and convalescent phase. G Pearson correlation matrix of indicated gene expression in IVIG-resistant patients during acute phase. H LRG1 levels in sera of healthy pediatric controls (n = 4), KD (KAWAKINRA screening visit data, n = 15), sJIA-associated MAS (n = 9), and MIS-C patients (n = 4). Data were analyzed by students’ unpaired (E) or ordinary one-way ANOVA followed by Sidak’s multiple comparison test (F). * = p < 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001