Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 6;13:5899. doi: 10.1038/s41467-022-33566-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Growth of chitinolytic Pseudoalteromonas spp. in the minimal medium supplemented with 0.2% (w/v) GlcNAc1A. The y-axis represents log₂ transformation of the OD₆₀₀ value. Data are presented as mean ± standard deviations (SD) (n = 2 independent experiments). b Genetic organization of the cdc cluster of P. prydzensis ACAM 620. c Heat map for the top ten most abundant proteins in the secretome of strain ACAM 620 grown on 0.5% (w/v) colloidal chitin as the sole carbon source. The colors in the heat map indicate relative protein abundance, ranging from high (red) to low abundance (blue). The data are presented as log₂ transformation of the mean values of two biological replicates for each protein. These ten proteins account for 83.86% of the total protein abundance. The locus tag, protein annotation, type of signal peptides and CAZy family (glycosyl hydrolase (GH), carbohydrate-binding module (CBM) and auxiliary activity (AA)) are shown. Chitinolytic enzymes encoded by the cdc cluster are marked by solid circles and the other one by an empty circle. SpI, signal peptidase I cleavage site; SpII, signal peptidase II cleavage site. d Positive-mode Q-TOF-MS spectrum of products generated by the AA10 LPMO from strain ACAM 620 acting on 0.2% (w/v) squid pen β-chitin in the presence of 1 mM AscA. The insets show the negative control reactions without either LPMO or AscA, which did not generate detectable amounts of oxidized chitooligosaccharides. 100% relative intensity in the inserts represents 9.1 ×10³ (control reaction without LPMO) and 7.0 ×10³ (control reaction without AscA) arbitrary units (a.u.), respectively. Theoretical masses of relevant products are listed in Supplementary Table 2. DP, degree of polymerization; subscript OX, C1-oxidized chitooligosaccharides with a GlcNAc1A moiety; DP3_ox-Ac, DP3_ox lacking one acetyl group; DP4_ox-Ac, DP4_ox lacking one acetyl group. The graph shows a representative MS spectrum of at least three independent replicates. Source data are provided as a Source Data file.