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. 2022 Oct 6;12:16788. doi: 10.1038/s41598-022-21110-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design with development of temperatures per treatment. Tanks were arranged in the depicted order vertically, with four tanks on every level. The experiment lasted 37 days, and temperatures were increased gradually from day 17 in all but the low nitrate (LN) control tanks. During the first 16 days of the experiment, both low nitrate treatments (LN and LN + W) were exposed to the same conditions. This changed as temperatures in the LN + W treatment increased together with the MN + W and HN + W treatments.