FIG. 2.

Deletion analysis of ITC1. (A) Linear diagram of Itc1p with a schematic representation of the deletion derivatives. The nuclear localization signal and leucine zipper domain within Itc1p are shown as solid and hatched boxes, respectively. Amino acid numbers from the initiation methionine are shown at the top. The bars show the approximate sizes of the truncated forms of Itc1p, with the number of amino acids (aa) at the right of each bar. (B) Suppression of the Ino⁻ phenotype of the Δhac1 strain by ITC1 deletion derivatives. HU1 harboring YCpL2-42 (HAC1), YEp133Δ1 (ITC1Δ1), YEp133Δ2 (ITC1Δ2), YEp133t (ITC1Δ), YEp133w (ITC1), or vector YEpM4 alone was 10-fold serially diluted and then spotted onto minimal medium containing histidine with or without inositol. The transformants were grown at 30°C for 3 days.