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Effects of overexpression of ITC1 and ISW2 on INO1 expression. The Δhac1 strain, HU1, harboring pHV-ADISW2 plus vector YEpM4 (ISW2), vector pHV-1 plus YEp133t (ITC1Δ), pHV-ADISW2 plus YEp133t (ISW2-ITC1Δ), vector pHV-1 plus YCpL2-42 (HAC1), or vector pHV-1 plus vector YEpM4 (vector) was 10-fold serially diluted and then spotted onto minimal medium with or without inositol. The transformants were grown at 30°C for 3 days.
