FIG. 2.

C-terminal truncation and internal deletion constructs of the C terminus of mcpA. The figure shows a diagram of the McpA protein present in the various chromosomal deletions of the mcpA gene in the named C. crescentus strains (Table 1). All McpA protein derivatives are membrane localized (data not shown). TM1 and TM2 denote the transmembrane domains; K1 and R1 denote the methylation domains. HCD denotes the highly conserved domain. The black boxes denote the extent of the deletions in the mcpA gene present in the named C. crescentus strains (Table 1). The amino acids in boldface are the mutated residues in the pXCP3ELD mutant. The black-boxed amino acid residues are the CheRB pentapeptide docking site. The plus and minus signs indicate whether the McpA derivative is degraded. The gray box labeled alpha helix denotes the predicted α-helix derived from the Tsr structure (17). The open box around the amino acid sequence denotes the extent of the requirement for McpA degradation.