FIG. 3.

Cell cycle immunoblots of the mcpA mutants. Cell cycle immunoblotting using McpA antisera was performed as described in Materials and Methods. All the proteins expressed from the mutant mcpA genes were membrane localized like the wild-type protein (data not shown). The strain MRKA800 is the wild-type strain where the full-length mcpA gene is under the control of the xylXp promoter. The other strains (Table 1) have various mcpA mutations under the control of the xylXp promoter (Fig. 2). The numbers above the cell cycle immunoblots represent the times in minutes at which samples were taken, and the C. crescentus drawings denote the progression through the cell cycle in PYEX medium. Cell cycle progression was monitored by microscopic analysis. Equal amounts of protein were loaded in each lane.