FIG. 4.

ClpX is required for the cell-cycle-controlled proteolysis of McpA. (A to C) McpA cell cycle immunoblots of strain UJ200 after 0, 3, and 6 h of xylose depletion. The swarmer cells from UJ200 were isolated after 0, 3, and 6 h of xylose depletion and then allowed to proceed through the cell cycle at 28°C in PYEG. Cell cycle progression was monitored by microscopic analysis. Equal amounts of protein were loaded in each lane. The numbers above the figure represent the times in minutes at which samples were taken. (D) Growth curves of the UJ200 strain after xylose depletion for 0, 3, 6, 9, and 12 h. Cell division occurred at 120 min for 0 h of depletion, 135 min for 3 h of depletion, and 180 min for 6 h of depletion.