FIG. 5.

The effects of ClpP depletion on McpA degradation. (A to E) McpA cell cycle immunoblots of strain UJ199 after 0, 3, 6, 9, and 12 h of xylose depletion. The swarmer cells from UJ199 were isolated after 0, 3, 6, 9, and 12 h of xylose depletion and then allowed to proceed through the cell cycle at 28°C in PYEG. Cell cycle progression was monitored by microscopic analysis. Equal amounts of protein were loaded in each lane. The numbers above the figure represent the times in minutes at which samples were taken. (F) Growth curves of the UJ199 strain after xylose depletion for 0, 3, 6, 9, and 12 h. Cell division occurred at 120 min for 0 h of depletion, 135 min for 3 h of depletion, and 150 min for 6 h of depletion. In the 9-h xylose depletion experiment, no cell division had occurred by 210 min.