TABLE 2.
Plasmids
| Plasmid | Descriptiona | Reference or source |
|---|---|---|
| pCHE22 | 2.29-kb EcoRI fragment of mcpA was cloned into pBluescript II SK(+) | 3 |
| pCHE22ΔS | 1.7 kb of SacI deletion of pCHE22 | This work |
| pSNX228-1 | Narrow-host-range pUC-based plasmid with novel MCS and M13 ori (pLITMUS28 [NEB]) and 0.6-kb HindIII-XhoI xylX fragment inserted into SnaBI site of pNTP228 | This work |
| pAS21 | Broad-host-range medium-copy vector with xylXp and MCS | A. Stotz and U. Jenal |
| pXCP1 | 2-kb HindIII fragment of pMCP6 was cloned into pAS21 | This work |
| pXCP2 | 2-kb HindIII fragment of pMCP6 was cloned into pSNX228-1 | This work |
| pXCP3 | BglII-EcoRV deletion of pXCP2 | This work |
| pXCP311 | Deletion of mcpA C terminus of pXCP3 | This work |
| pXCP313 | Deletion of mcpA C terminus of pXCP3 | This work |
| pXCP305 | Deletion of mcpA C terminus of pXCP3 | This work |
| pXCP307 | Deletion of mcpA C terminus of pXCP3 | This work |
| pXCP382 | Internal deletion of mcpA of pXCP3 | This work |
| pXCP384 | Internal deletion of mcpA of pXCP3 | This work |
| pXCP3ELD | ELD mutant of pXCP3 | This work |
a
Abbreviations: MCS, multiple cloning site; NEB, New England Biolabs..