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. 2022 Oct 6;13:5688. doi: 10.1038/s41467-022-33364-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Images of SNR-derived organoids at day 49, 83, and 111 post SNR isolation. b Quantification of organoid sizes over time (n = 47 [day19], 46 [day25], 22 [day32], 25 [day49], 26 [day83], 26 [day111] and 25 [day146] organoids produced in two differentiation batches from H9 line). c Quantification of sizes of 5-month-old SNR-derived organoids produced from different stem cell lines (n = 17, 15, 38, 15 organoids produced in 2–4 differentiation batches from 2242-5, EP2-15, H9, and EYQ2-20 lines, respectively). d–f Image of a 5-month-old SNR-derived organoid (d) and 350-μm-thick organoid slice (f) in comparison with E14.5 mouse brain (e) and coronal mouse brain hemisphere slice (f). g UMAP visualizations of unsupervised clustering of cells obtained from 5-month-old SNR-derived organoids generated from different stem cell lines. The clustering was performed on the combined dataset. h Percentages of cells from different organoids in different clusters. i UMAP visualization of combined 22,486 cells from six organoids. j Heatmap visualizations of expression of selected cell type-specific genes. k Heatmap of Pearson correlation between cell clusters identified in 5-month-old organoids and those in primary human fetal tissue from Nowakowski et al.⁷. Data are mean ± s.e.m. Scale bars = 1 mm. RG radial glia, IP intermediate neural progenitor, EN excitatory neuron, IN inhibitory neuron, CTX cortex, OPC oligodendrocyte progenitor cell, U unknown. Source data are provided as a Source Data file.