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. 2022 Oct 6;13:5688. doi: 10.1038/s41467-022-33364-z

Fig. 6. Diversity of neural cells, including outer radial glia, astrocytes, and ependymal- and periendothelial-like cells are generated in 5-month-old SNR-derived organoids.

Fig. 6

a Organoid section immunostained for cleaved caspase-3. b Percentage and distribution of cleaved caspase 3-expressing cells in organoid sections (n = 4 sections from two organoids produced in two batches from 2242-5 and H9 lines). c, d, f–h Organoid sections immunostained for PAX6 (c), TUJ1 and GFAP (d), FOXJ1 and S100β (f), O4 and MBP (g), and PDGFR-β and α-SMA (h). e Percentages of cells expressing cell type-specific markers (n = 6 [PAX6], 4 [MAP2], 4 [GFAP], 4 [O4] and 4 [MBP] organoids/1–3 sections per organoid produced in 1–3 batches from 2242-5, EP2-15, and H9 lines). Data are mean ± s.e.m. Scale bars = 500 (a), 200 and 20 (zoom-in) μm (c, d, f–h). L lumen. Source data are provided as a Source Data file.