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. 2022 Oct 6;13:5688. doi: 10.1038/s41467-022-33364-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Heatmap visualizations of selected neuronal markers. b, c Organoid sections immunostained for TBR1, and SATB2 (b) and CTIP2, and CUX1 (c) and relative distributions of cells expressing these markers the marked ROIs. d Percentages of cells expressing cell different neuron type-specific markers (n = 8 [CTIP2], 5 [TBR1], 6 [SATB2], 5 [CUX1] and 5 [GABA/GAD67] organoids/1–2 sections per organoid produced in 2–3 batches from 2242-5 and EP2-15 lines). e Organoid sections immunostained for Calretinin (CALR) and GAD67; Calbindin (CALB) and GABA; Somatostatin (SST) and GABA; Parvalbumin (PV) and GABA; and Vasoactive intestinal peptide (VIP) and GAD67. f Percentages of inhibitory neurons expressing different interneuron markers (n = 4 organoids/1–3 sections per organoid, produced in four batches from 2242-5, EP2-15, H9, and EYQ2-20 lines). Data are presented as mean ± s.e.m. Scale bars = 200 and 20 (zoom-in) (b, c) and 50 (e) μm. Source data are provided as a Source Data file.