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. 2022 Oct 6;12:16732. doi: 10.1038/s41598-022-20488-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Schematic drawing of the Y-maze used for ACP two-choice behavioral assays. Behavioral preference index of ACP to humid air at the indicated concentrations versus dry air, and dry air (dry/dry) or moist air at 75% (wet/wet) pumped into both arms as controls. N = 5–12 trials of 20 male psyllids for each dose. Error bars indicate s.e.m.; ** p < 0.01; *** p < 0.001. (B) Behavioral preference index of ACP to dry air (dry/dry, Left) or moist air at 75% (wet/wet, Right) pumped into both arms as controls and wet or dry air versus wet or dry air with 1% pentylamine. n = 6–12 trials of 20 male psyllids for each humidity level. Error bars indicate s.e.m.; ***p < 0.001. (C) Schematic representation of two-choice mosquito oviposition assay. (D) Left. Oviposition preference index in Aedes aegypti mosquitoes to the indicated concentration of pentylamine versus water alone. n = 4–6 trials with 15 mosquitoes per trial. Error bars represent s.e.m. *p < 0.05. Right. Oviposition preference index in Anopheles mosquitoes to the indicated concentration of pentylamine versus water alone. n = 4 and 6 trials respectively, with 15 mosquitoes/trial. Error bars represent s.e.m. *p < 0.05.