Table 2.

Inhibition data of carbonic anhydrase enzyme for series (A) target compounds and standard inhibitor, AAZ, against hCAs I, II, IX and XII via a stopped-flow CO₂ hydrase assay³⁷.

Comp	R	R1	KI (nM)*
			hCA I	hCA II	hCA IX	hCA XII
10	–	–	72.3	49.2	2454	7633
12a	H	H	3599	435.9	1536	8057
12b	H	Br	2616	2002	1022	8208
12c	OCH3	H	961.9	1937	1393	8678
12d	N(CH3)2	H	577.3	363.6	190.0	187.9
12e	–OCH2O–		4052	893.0	971.3	8537
12f.	F	H	589.6	493.4	194.8	4198
12g	CH3	H	158.7	696.9	194.8	2306
12h	H	Cl	507.8	320.0	52.2	87.5
12i	H	F	480.1	253.1	38.8	181.7
12j	H	OCH3	494.2	365.7	89.9	83.7
AAZ	–	–	250	12.1	25.7	5.7

*Mean from 3 different assays, by a stopped flow technique (errors were in the range of ± 5–10% of the reported values).