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. 2022 Oct 6;13:5879. doi: 10.1038/s41467-022-33662-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Schematic of WT and mutant CPSF6_1-358 constructs. Amino acid sequences of the CPSF6(LCR-FG-LCR) segment targeted by mutagenesis are shown. CPSF6₃₅₈/WT: native Pro residues are in bold and underlined. The FG containing peptide is highlighted in green. CPSF6₃₅₈(ΔFG): FG residues were deleted. CPSF6₃₅₈/mP-FGp: Pro residues in the FG-containing peptide were substituted with Gly or Ser. CPSF6₃₅₈/mP-LCR: Pro residues in the LCR segments were substituted with non-charged residues. CPSF6₃₅₈/mP-ED: Pro residues in the LCR segments were substituted with charged residues Glu or Asp. CPSF6₃₅₈/NE: the LCR segments of CPSF6₃₅₈ were replaced with non-LCR counterparts from NEURM_97-179. CPSF6₃₅₈/FU: the LCR segments of CPSF6₃₅₈ were replaced with alternative LCR from FUS_35-117. CPSF6₃₅₈(ΔFG)/FU: FG residues were deleted from CPSF6₃₅₈/FU. b Relative infectivity of VSV-G pseudotyped HIV-1 in the Hela cells stably overexpressing proteins shown in a. Infectivity was normalized to CPSF6₃₅₈(ΔFG). The averaged data (+/− SD) from three independent experiments are shown. Source data are provided as a Source Data file.