Table 1.

Primers used in this study.

Pathogen	Target gene	Primer name	Sequence	Size (bp)	Reference
Rickettsia spp.	17-kD	R-r17k1-F	TTTACAAAATTCTAAAAACCAT	539	(Ishikura et al., 2003)
		R-r17k539-R	TCAATTCACAACTTGCCATT
		R-r17k90-F	GCTCTTGCAACTTCTATGTT	450
		R-r17k539-R	TCAATTCACAACTTGCCATT
	ompA	R-r190k.71p	TGGCGAATATTTCTCCAAAA	650	(Ishikura et al., 2003)
		R-r190k.720n	TGCATTTGTATTACCTATTGT
		R-r190k.71p	TGGCGAATATTTCTCCAAAA	532
		R-r190k.602n	AGTGCAGCATTCGCTCCCCCT
	ompB	Rc.rompB.4362p	GTCAGCGTTACTTCTTCGATGC	475	(Choi et al., 2005)
		Rc.rompB.4,836n	CCGTACTCCATCTTAGCATCAG
		Rc.rompB.4,496p	CCAATGGCAGGACTTAGCTACT	267
		Rc.rompB.4,762n	AGGCTGGCTGATACACGGAGTAA
	sca4	R-sca4-1072F	TCGGTTGAACCACCTCAGCATA	711	(Li et al., 2020)
		R-sca4-1782R	TGTGCCGGACTGAGAACTTGGA
		R-sca4-1146F	GGCTTCACAAATGCCACAGTCG	389
		R-sca4-1154R	TCTGCTGTTTTTGCTGCGGCTC
Rickettsia felis	gltA	Rfelis-gltA-F1	GCAAGTATTGGTGAGGATGTAATC	886	(Hii et al., 2011)
		Rfelis-gltA-R1	CTGCGGCACGTGGGTCATAG
		Rfelis-gltA-F2	GCGACATCGAGGATATGACAT	654
		Rfelis-gltA-R2	GGAATATTCTCAGAACTACCG
	felB*	RfelB-F	TAATTTTAACGGAACAGACGGT	97	(Phomjareet et al., 2020)
		RfelB-R	GCCTAAACTTCCTGTAACATTAAAG
		RfelB-P	FAM-TGCTGCTGGTGGCGGTGCTA-BHQ

*Real-time PCR method.