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. 2022 Sep 23;9:1007064. doi: 10.3389/fmolb.2022.1007064

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Copyright © 2022 Sun, Soans, Mishina, Petricci, Pommier, Nitiss and Nitiss.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of MRE11 endonuclease activity results in accumulation of etoposide-induced TOP2/DNA complexes. RH30 cells were pre-treated with 25 μM PFM01, 25 μM PFM03, or 25 μM PFM X for 4 h. Etoposide (10 μM) was added, and incubation was continued for 2 h. Cells were then harvested for ICE assays. (A) Representative ICE assay measuring the levels of TOP2αcc (B) Densitometric analysis of all assays comparing relative integrated densities of TOP2αcc signals. Integrated density of TOP2αcc signal of each group was normalized to that of cells treated with etoposide alone. (C) Representative ICE assay measuring the levels of TOP2βcc. (D) Densitometric analysis of all assays comparing relative integrated densities of TOP2βcc signals. Integrated density of TOP2βcc signal of each group was normalized to that of cells treated with etoposide alone.