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. 2022 Sep 23;9:1007064. doi: 10.3389/fmolb.2022.1007064

FIGURE 5.

FIGURE 5

Inhibition of MRE11 endonuclease activity does not change the level of TOP2 covalent complexes when MRE11 is depleted. RH30 cells were transfected with non-targeting siRNA control (siControl) or siRNA targeting MRE11 (siMRE11) as in Figure 1. These cells were then pre-treated with 25 μM PFM03 where indicated. Etoposide (10 μM) was added, and incubation was continued for 2 h before ICE assays. (A) Representative ICE assay measuring the levels of TOP2αcc. (B) Densitometric analysis of all assays comparing relative integrated densities of TOP2αcc signals. Integrated density of TOP2αcc signal of each group was normalized to that of siControl cells treated with etoposide alone. Levels of TOP2αcc for siControl versus siMre11 cells treated with PFM03 were not statistically different. (C) Representative ICE assay measuring the levels of TOP2βcc. (D) Densitometric analysis of all assays comparing relative integrated densities of TOP2βcc signals.